Bulk mRNA-sequencing data of the estrogen and androgen responses in the human prostate cancer cell line VCaPGEO)

Abstract

Prostate cancer is a hormone-dependent disease that relies on the androgen signaling, as well as on the estrogen signaling, for growth and survival. To identify the genes regulated by these sex-steroid hormones in the human prostate cancer cell line VCaP, these cells were treated for 24 h with either androgens and/or estrogens. Then, the RNA of each sample was purified for sequencing to generate bulk mRNA-seq data. After verifying raw quality, reads were pseudo-aligned on the human reference transcriptome (Gencode v27). Analysis was carried out on aligned and quantified data to determine the transcriptomic changes following each hormonal treatment. These data presented herein can be reanalyzed with specific fold-change thresholds for gene expression, or with different pair-wise combinations to compare the hormones’ transcriptional impacts on VCaP cells and better understand prostate cancer cell biology.

Keywords

• Steroids
• Estrogen receptor
• Androgen receptor
• SERMs
• Tamoxifen
• Benign prostate hyperplasia