MicroRNA-181a* Targets Nanog in a Subpopulation of CD34+ Cells Isolated From Peripheral Blood

Paul J Mintz; Pål Sætrom; Vikash Reebye; Marie B Lundbæk; Kaiqin Lao; John J Rossi; Karin ML Gaensler; Noriyuki Kasahara; Joanna P Nicholls; Steen Jensen; Abdelali Haoudi; Mohamed M Emara; Myrtle YA Gordon; Nagy A Habib

doi:10.1038/mtna.2012.29

Molecular Therapy: Nucleic Acids (Jan 2012)

MicroRNA-181a* Targets Nanog in a Subpopulation of CD34+ Cells Isolated From Peripheral Blood

Paul J Mintz,
Pål Sætrom,
Vikash Reebye,
Marie B Lundbæk,
Kaiqin Lao,
John J Rossi,
Karin ML Gaensler,
Noriyuki Kasahara,
Joanna P Nicholls,
Steen Jensen,
Abdelali Haoudi,
Mohamed M Emara,
Myrtle YA Gordon,
Nagy A Habib

Affiliations

Paul J Mintz: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
Pål Sætrom: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
Vikash Reebye: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
Marie B Lundbæk: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
Kaiqin Lao: Genetic Systems, Applied Biosystems, Part of Life Technologies, Foster City, California, USA
John J Rossi: Division of Molecular Biology, Beckman Research Institute of City of Hope, Duarte, California, USA
Karin ML Gaensler: Department of Medicine, University of California, San Francisco, California, USA
Noriyuki Kasahara: Department of Medicine, UCLA School of Medicine, Los Angeles, California, USA
Joanna P Nicholls: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
Steen Jensen: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
Abdelali Haoudi: Qatar Biomedical Research Institute, Education City, Doha, Qatar
Mohamed M Emara: Qatar Biomedical Research Institute, Education City, Doha, Qatar
Myrtle YA Gordon: Department of Hematology, Faculty of Medicine, Imperial College London, London, UK
Nagy A Habib: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK

DOI: https://doi.org/10.1038/mtna.2012.29
Journal volume & issue: Vol. 1, no. C

Abstract

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Exploiting the properties of stem cells by microRNA (miRNA) profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC), the targets corresponding to many of these miRNA have not yet been fully elucidated. By miRNA profiling in a subpopulation of CD34+ cells isolated from peripheral blood, we have identified eight clusters of miRNA that were differentially expressed. Further analysis of one of the clusters by bioinformatics revealed that a miRNA, miR-181a*, which is highly expressed in the adherent CD34+ cells, affects the expression levels of Nanog, a stem cell surrogate marker. We show specifically by reporter assay and mutational analysis that miR-181a* targets a seedless 3′ compensatory site in the 3′UTR of Nanog and affects gene expression. We demonstrate that inhibiting miR-181a* upregulates the Nanog expression level, in addition to an increase in alkaline phosphatase activity. Our studies suggest that miR-181a* may be important in controlling the expression level of Nanog in a subpopulation of CD34+ cells.

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

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