Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing

Chirag Nepal; Yavor Hadzhiev; Piotr Balwierz; Estefanía Tarifeño-Saldivia; Ryan Cardenas; Joseph W. Wragg; Ana-Maria Suzuki; Piero Carninci; Bernard Peers; Boris Lenhard; Jesper B. Andersen; Ferenc Müller

doi:10.1038/s41467-019-13687-0

Nature Communications (Jan 2020)

Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing

Chirag Nepal,
Yavor Hadzhiev,
Piotr Balwierz,
Estefanía Tarifeño-Saldivia,
Ryan Cardenas,
Joseph W. Wragg,
Ana-Maria Suzuki,
Piero Carninci,
Bernard Peers,
Boris Lenhard,
Jesper B. Andersen,
Ferenc Müller

Affiliations

Chirag Nepal: Biotech Research and Innovation Centre, Department of Health and Medical Sciences, University of Copenhagen
Yavor Hadzhiev: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Piotr Balwierz: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Estefanía Tarifeño-Saldivia: Laboratory for Molecular Biology and Genetic Engineering, GIGA-R, Université de Liège
Ryan Cardenas: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Joseph W. Wragg: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Ana-Maria Suzuki: Division of Genomic Technologies, RIKEN Center for Life Science Technologies
Piero Carninci: Division of Genomic Technologies, RIKEN Center for Life Science Technologies
Bernard Peers: Laboratory for Molecular Biology and Genetic Engineering, GIGA-R, Université de Liège
Boris Lenhard: Institute of Clinical Sciences, Faculty of Medicine, Imperial College London
Jesper B. Andersen: Biotech Research and Innovation Centre, Department of Health and Medical Sciences, University of Copenhagen
Ferenc Müller: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

DOI: https://doi.org/10.1038/s41467-019-13687-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 16

Abstract

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The functional significance of start site choice in promoter architectures is little understood. Here the authors identify in zebrafish development and mammalian cells a class of dual-initiation promoters, in which non-canonical YC dinucleotides reflecting 5’-TOP/TCT initiation are intertwined with canonical YR-initiation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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