Frontiers in Plant Science (Jul 2023)
Comparative cytological and transcriptome analyses of ny2 mutant delayed degeneration of tapetal cells and promotes abnormal microspore development in neo-tetraploid rice
- Nabieu Kamara,
- Nabieu Kamara,
- Nabieu Kamara,
- Nabieu Kamara,
- Nabieu Kamara,
- Yamin Jiao,
- Yamin Jiao,
- Yamin Jiao,
- Yamin Jiao,
- Weicong Huang,
- Weicong Huang,
- Weicong Huang,
- Weicong Huang,
- Lichong Cao,
- Lichong Cao,
- Lichong Cao,
- Lichong Cao,
- Lianjun Zhu,
- Lianjun Zhu,
- Lianjun Zhu,
- Lianjun Zhu,
- Chongchong Zhao,
- Chongchong Zhao,
- Chongchong Zhao,
- Chongchong Zhao,
- Xu Huang,
- Xu Huang,
- Xu Huang,
- Xu Huang,
- Fimanekeni Ndaitavela Shivute,
- Fimanekeni Ndaitavela Shivute,
- Fimanekeni Ndaitavela Shivute,
- Fimanekeni Ndaitavela Shivute,
- Fimanekeni Ndaitavela Shivute,
- Xiangdong Liu,
- Xiangdong Liu,
- Xiangdong Liu,
- Xiangdong Liu,
- Jinwen Wu,
- Jinwen Wu,
- Jinwen Wu,
- Jinwen Wu,
- Muhammad Qasim Shahid,
- Muhammad Qasim Shahid,
- Muhammad Qasim Shahid,
- Muhammad Qasim Shahid
Affiliations
- Nabieu Kamara
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Nabieu Kamara
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Nabieu Kamara
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Nabieu Kamara
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Nabieu Kamara
- Crop Improvement Programme, Rokupr Agricultural Research Center, Rokupr - Sierra Leone Agricultural Research Institute (SLARI), Freetown, Sierra Leone
- Yamin Jiao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Yamin Jiao
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Yamin Jiao
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Yamin Jiao
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Weicong Huang
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Weicong Huang
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Weicong Huang
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Weicong Huang
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Lichong Cao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Lichong Cao
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Lichong Cao
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Lichong Cao
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Lianjun Zhu
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Lianjun Zhu
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Lianjun Zhu
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Lianjun Zhu
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Chongchong Zhao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Chongchong Zhao
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Chongchong Zhao
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Chongchong Zhao
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Xu Huang
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Xu Huang
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Xu Huang
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Xu Huang
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Fimanekeni Ndaitavela Shivute
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Fimanekeni Ndaitavela Shivute
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Fimanekeni Ndaitavela Shivute
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Fimanekeni Ndaitavela Shivute
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Fimanekeni Ndaitavela Shivute
- Multi-disciplinary Research Services, University of Namibia, Windhoek, Namibia
- Xiangdong Liu
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Xiangdong Liu
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Xiangdong Liu
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Xiangdong Liu
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Jinwen Wu
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Jinwen Wu
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Jinwen Wu
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Jinwen Wu
- College of Agriculture, South China Agricultural University, Guangzhou, China
- Muhammad Qasim Shahid
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Muhammad Qasim Shahid
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Muhammad Qasim Shahid
- Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China
- Muhammad Qasim Shahid
- College of Agriculture, South China Agricultural University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2023.1229870
- Journal volume & issue
-
Vol. 14
Abstract
We aimed to investigate the genetic defects related to pollen development and infertility in NY2, a novel tetraploid rice germplasm known as Neo-tetraploid rice. This rice variety was created through the crossbreeding and selective breeding of various autotetraploid rice lines and has previously shown high fertility. Our previous research has revealed that the NY2 gene, encoding a eukaryotic translation initiation factor 3 subunit E, regulates pollen fertility. However, the underlying mechanism behind this fertility is yet to be understood. To shed light on this matter, we performed a combined cytological and transcriptome analysis of the NY2 gene. Cytological analysis indicated that ny2 underwent abnormal tapetal cells, microspore, and middle layer development, which led to pollen abortion and ultimately to male sterility. Genetic analysis revealed that the F1 plants showed normal fertility and an obvious advantage for seed setting compared to ny2. Global gene expression analysis in ny2 revealed a total of 7545 genes were detected at the meiosis stage, and 3925 and 3620 displayed upregulation and downregulation, respectively. The genes were significantly enriched for the gene ontology (GO) term “carbohydrate metabolic process. Moreover, 9 genes related to tapetum or pollen fertility showed down-regulation, such as OsABCG26 (ATP Binding Cassette G26), TMS9-1 (Thermosensitive Male Sterility), EAT1 (Programmed cell death regulatory), KIN14M (Kinesin Motor), OsMT1a (Metallothionein), and OsSTRL2 (Atypical strictosidine synthase), which were validated by qRT-PCR. Further analyses of DEGs identified nine down-regulated transcription factor genes related to pollen development. NY2 is an important regulator of the development of tapetum and microspore. The regulatory gene network described in this study may offer important understandings into the molecular processes that underlie fertility control in tetraploid rice.
Keywords