Enhancement of prime editing via xrRNA motif-joined pegRNA

Guiquan Zhang; Yao Liu; Shisheng Huang; Shiyuan Qu; Daolin Cheng; Yuan Yao; Quanjiang Ji; Xiaolong Wang; Xingxu Huang; Jianghuai Liu

doi:10.1038/s41467-022-29507-x

Nature Communications (Apr 2022)

Enhancement of prime editing via xrRNA motif-joined pegRNA

Guiquan Zhang,
Yao Liu,
Shisheng Huang,
Shiyuan Qu,
Daolin Cheng,
Yuan Yao,
Quanjiang Ji,
Xiaolong Wang,
Xingxu Huang,
Jianghuai Liu

Affiliations

Guiquan Zhang: State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center at Medical School of Nanjing University
Yao Liu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University
Shisheng Huang: Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
Shiyuan Qu: Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
Daolin Cheng: State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center at Medical School of Nanjing University
Yuan Yao: Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University
Quanjiang Ji: School of Physical Science and Technology, ShanghaiTech University
Xiaolong Wang: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University
Xingxu Huang: Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
Jianghuai Liu: State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center at Medical School of Nanjing University

DOI: https://doi.org/10.1038/s41467-022-29507-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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The prime editors (PEs) have shown great promise for precise genome modification. Here the authors place a stabilizing viral xrRNA motif to the 3′ of pegRNAs to enhance editing efficiencies.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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