DNA fingerprinting and diversity analysis in Aus genotypes using microsatellite markers

Abstract

DNA fingerprinting and genetic diversity of 94 Aus (6 BRRI released Aus variety and 88 local Aus landraces) genotypes were carried out to protect the Aus landraces from biopiracy. A total of 91 microsatellite markers were tested for screening the genotypes. Among 91 amplified products, 56% have polymorphic bands giving 195 alleles. The number of alleles per locus ranged from four (RM25 and RM147) to twenty seven (RM519), where average allele number was 9.76. The Polymorphism Information Contents (PIC) lied between 0.455 (RM5) to 0.934 (RM519). Most robust marker was found RM519 since it provided the highest PIC value (0.934). Pair-wise genetic dissimilarity co-efficient showed the lowest genetic dissimilarity was found BRRI dhan42 and BRRI dhan43 and the highest genetic dissimilarity was found local landraces each other. Here it is shown that most Aus landraces is recognized to have broad genetic base. Thus it is recommended to use these landraces for future breeding program or include new and untouched local landraces to incorporate new genes and broaden genetic base.

Keywords

• Aus rice
• genetic diversity
• microsatellite markers
• DNA fingerprinting