Journal of Materials Science: Materials in Medicine (Jul 2023)

A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro

  • Yong Mao,
  • Nikita John,
  • Nicole M. Protzman,
  • Desiree Long,
  • Raja Sivalenka,
  • Shamshad Azimi,
  • Brandon Mirabile,
  • Robert Pouliot,
  • Anna Gosiewska,
  • Robert J. Hariri,
  • Stephen A. Brigido

DOI
https://doi.org/10.1007/s10856-023-06740-4
Journal volume & issue
Vol. 34, no. 7
pp. 1 – 14

Abstract

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Abstract Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity. Graphical Abstract