Nature Communications (Sep 2018)
Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes
- Gabriel B. Ferguson,
- Ben Van Handel,
- Maxwell Bay,
- Petko Fiziev,
- Tonis Org,
- Siyoung Lee,
- Ruzanna Shkhyan,
- Nicholas W. Banks,
- Mila Scheinberg,
- Ling Wu,
- Biagio Saitta,
- Joseph Elphingstone,
- A. Noelle Larson,
- Scott M. Riester,
- April D. Pyle,
- Nicholas M. Bernthal,
- Hanna KA Mikkola,
- Jason Ernst,
- Andre J. van Wijnen,
- Michael Bonaguidi,
- Denis Evseenko
Affiliations
- Gabriel B. Ferguson
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Ben Van Handel
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Maxwell Bay
- Department of Stem Cell Research and Regenerative Medicine, USC
- Petko Fiziev
- Bioinformatics Interdepartmental Program, UCLA
- Tonis Org
- Department of Molecular, Cell and Developmental Biology, UCLA
- Siyoung Lee
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Ruzanna Shkhyan
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Nicholas W. Banks
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Mila Scheinberg
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Ling Wu
- InVitro Cell Research, LLC
- Biagio Saitta
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Joseph Elphingstone
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- A. Noelle Larson
- Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Center of Regenerative Medicine, Mayo Clinic
- Scott M. Riester
- Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Center of Regenerative Medicine, Mayo Clinic
- April D. Pyle
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA
- Nicholas M. Bernthal
- Department of Orthopaedic Surgery, David Geffen School of Medicine, UCLA
- Hanna KA Mikkola
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA
- Jason Ernst
- Department of Biological Chemistry, UCLA
- Andre J. van Wijnen
- Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Center of Regenerative Medicine, Mayo Clinic
- Michael Bonaguidi
- Department of Stem Cell Research and Regenerative Medicine, USC
- Denis Evseenko
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- DOI
- https://doi.org/10.1038/s41467-018-05573-y
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 16
Abstract
Human development provides a roadmap for advancing pluripotent stem cell-based regenerative therapies. Here the authors mapped human skeletogenesis using RNA sequencing on 5 cell types from a single foetal stage as well as chondrocytes at 4 stages in vivo and 2 stages during in vitro differentiation.
