Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus

Nadezhda B. Rudometova; Anastasia A. Fando; Lyubov A. Kisakova; Denis N. Kisakov; Mariya B. Borgoyakova; Victoria R. Litvinova; Vladimir A. Yakovlev; Elena V. Tigeeva; Danil I. Vahitov; Sergey V. Sharabrin; Dmitriy N. Shcherbakov; Veronika I. Evseenko; Ksenia I. Ivanova; Andrei S. Gudymo; Tatiana N. Ilyicheva; Vasiliy Yu. Marchenko; Alexander A. Ilyichev; Andrey P. Rudometov; Larisa I. Karpenko

doi:10.3390/vaccines12020143

Vaccines (Jan 2024)

Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus

Nadezhda B. Rudometova,
Anastasia A. Fando,
Lyubov A. Kisakova,
Denis N. Kisakov,
Mariya B. Borgoyakova,
Victoria R. Litvinova,
Vladimir A. Yakovlev,
Elena V. Tigeeva,
Danil I. Vahitov,
Sergey V. Sharabrin,
Dmitriy N. Shcherbakov,
Veronika I. Evseenko,
Ksenia I. Ivanova,
Andrei S. Gudymo,
Tatiana N. Ilyicheva,
Vasiliy Yu. Marchenko,
Alexander A. Ilyichev,
Andrey P. Rudometov,
Larisa I. Karpenko

Affiliations

Nadezhda B. Rudometova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Anastasia A. Fando: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Lyubov A. Kisakova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Denis N. Kisakov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Mariya B. Borgoyakova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Victoria R. Litvinova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Vladimir A. Yakovlev: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Elena V. Tigeeva: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Danil I. Vahitov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Sergey V. Sharabrin: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Dmitriy N. Shcherbakov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Veronika I. Evseenko: Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia
Ksenia I. Ivanova: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Andrei S. Gudymo: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Tatiana N. Ilyicheva: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Vasiliy Yu. Marchenko: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Alexander A. Ilyichev: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Andrey P. Rudometov: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia
Larisa I. Karpenko: Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia

DOI: https://doi.org/10.3390/vaccines12020143
Journal volume & issue: Vol. 12, no. 2
p. 143

Abstract

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In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus—the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy.

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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