Genetic Dissection of Tiller Number <i>qTN4</i> in Rice

Hongmei Chen; Yongyi Fan; Dian Li; Zhihao Sun; Zheyan Ruan; Huali Yang; Yiwei Kang; Weixun Wu; Qunen Liu; Xiaodeng Zhan; Liyong Cao; Ming Zhou; Shihua Cheng; Yingxin Zhang

doi:10.3390/agriculture13020411

Agriculture (Feb 2023)

Genetic Dissection of Tiller Number <i>qTN4</i> in Rice

Hongmei Chen,
Yongyi Fan,
Dian Li,
Zhihao Sun,
Zheyan Ruan,
Huali Yang,
Yiwei Kang,
Weixun Wu,
Qunen Liu,
Xiaodeng Zhan,
Liyong Cao,
Ming Zhou,
Shihua Cheng,
Yingxin Zhang

Affiliations

Hongmei Chen: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Yongyi Fan: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Dian Li: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Zhihao Sun: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Zheyan Ruan: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Huali Yang: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Yiwei Kang: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Weixun Wu: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Qunen Liu: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Xiaodeng Zhan: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Liyong Cao: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Ming Zhou: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
Shihua Cheng: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
Yingxin Zhang: State Key Laboratory of Rice Biology and Breeding and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China

DOI: https://doi.org/10.3390/agriculture13020411
Journal volume & issue: Vol. 13, no. 2
p. 411

Abstract

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Tiller number (TN) is an important trait that contributes to yield in rice (Oryza sativa L.). A set of 76 chromosome segment substitution lines (CSSLs) derived from the super-hybrid cross between Zhonghui 9308 (ZH9308) and Xieqingzao B (XQZB) was used to map quantitative trait loci (QTL) controlling tiller number (TN). A total of four QTLs were detected in Fuyang, Zhejiang Province (30.15° N, 120° E). Two QTLs were detected in Lingshui, Hainan Province (18.5° N, 110° E) in our previous study. To further map the QTL on chromosome 4, namely qTN4, the line CSSL29 with a lower tiller number was selected to cross with ZH9308 to develop the secondary F2 population. In the F2:3 population, the qTN4 was validated and subsequently narrowed down to a 4.08 Mb region. What is more, combined phenotype with genotype, qTN4 was dissected into two QTLs, qTN4.1 and qTN4.2, in the F4:5 population. The qTN4.1 and qTN4.2 explained 34.31% and 32.05% of the phenotypic variance, with an additive effect of 1.47 and 1.38, respectively. Finally, the qTN4.1 and qTN4.2 were fine-mapped into a 193.55 Kb and 175.12 Kb intervals on chromosome 4, respectively. Based on genotype and phenotype, four near-isogenic lines (NILs) were selected in the mapping populations. Compared with NILCSSL29, tiller number (TN), grain setting rate, grain length (GL), the ratio of grain length to width (LWR) and grain yield per plant of NILZH9308, NIL-qTN4.1ZH9308 and NIL-qTN4.2ZH9308 were increased, and the heading date of these three lines were earlier than that of NILCSSL29. Interestingly, among the candidate genes of qTN4.1 and qTN4.2, except for LOC_Os04g23550, none of the other genes has been cloned, indicating the existence of a novel gene-controlling tiller number. These results lay a foundation for the analysis of QTL controlling tiller number in ZH9308 and provide a theoretical basis for the application of ZH9308 in super-hybrid breeding.

Published in Agriculture

ISSN: 2077-0472 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Agriculture (General)
Website: http://www.mdpi.com/journal/agriculture

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