Ultrasound-triggered biomimetic ultrashort peptide nanofiber hydrogels promote bone regeneration by modulating macrophage and the osteogenic immune microenvironment

Fan Zhang; Mingchen Lv; Siyuan Wang; Mengyao Li; Yu Wang; Congjiao Hu; Wei Hu; Xuekui Wang; Xiaogang Wang; Zhiduo Liu; Zhen Fan; Jianzhong Du; Yao Sun

Bioactive Materials (Jan 2024)

Ultrasound-triggered biomimetic ultrashort peptide nanofiber hydrogels promote bone regeneration by modulating macrophage and the osteogenic immune microenvironment

Fan Zhang,
Mingchen Lv,
Siyuan Wang,
Mengyao Li,
Yu Wang,
Congjiao Hu,
Wei Hu,
Xuekui Wang,
Xiaogang Wang,
Zhiduo Liu,
Zhen Fan,
Jianzhong Du,
Yao Sun

Affiliations

Fan Zhang: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China
Mingchen Lv: Department of Gynaecology and Obstetrics, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 201804, China
Siyuan Wang: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China
Mengyao Li: Department of Immunology and Microbiology, The Minister of Education Key Laboratory of Cell Death and Differentiation, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, China
Yu Wang: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China
Congjiao Hu: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China
Wei Hu: Department of Gynaecology and Obstetrics, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 201804, China
Xuekui Wang: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China
Xiaogang Wang: Key Laboratory of Big Data-Based Precision Medicine, School of Engineering Medicine, Beihang University, China
Zhiduo Liu: Department of Immunology and Microbiology, The Minister of Education Key Laboratory of Cell Death and Differentiation, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, China
Zhen Fan: Department of Gynaecology and Obstetrics, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 201804, China; Corresponding author. Department of Gynaecology and Obstetrics, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
Jianzhong Du: Department of Gynaecology and Obstetrics, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 201804, China; Corresponding author. Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, 201804, China.
Yao Sun: Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 200072, China; Corresponding author.

Journal volume & issue: Vol. 31
pp. 231 – 246

Abstract

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The immune microenvironment plays a vital role in bone defect repair. To create an immune microenvironment that promotes osteogenesis, researchers are exploring ways to enhance the differentiation of M2-type macrophages. Functional peptides have been discovered to effectively improve this process, but they are limited by low efficiency and rapid degradation in vivo. To overcome these issues, peptide with both M2 regulatory and self-assembly modules was designed as a building block to construct an ultrasound-responsive nanofiber hydrogel. These nanofibers can be released from hydrogel in a time-dependent manner upon ultrasound stimulation, activating mitochondrial glycolytic metabolism and the tricarboxylic acid cycle, inhibiting reactive oxygen species production and enhancing M2 macrophage polarization. The hydrogel exhibits advanced therapeutic potential for bone regeneration by triggering M2 macrophages to secrete BMP-2 and IGF-I, accelerating the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts. Thus, modularly designed biomimetic ultrashort peptide nanofiber hydrogels provide a novel strategy to rebuild osteogenic immune microenvironments for bone repair.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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