A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

Joep Beumer; Maarten H. Geurts; Mart M. Lamers; Jens Puschhof; Jingshu Zhang; Jelte van der Vaart; Anna Z. Mykytyn; Tim I. Breugem; Samra Riesebosch; Debby Schipper; Petra B. van den Doel; Wim de Lau; Cayetano Pleguezuelos-Manzano; Georg Busslinger; Bart L. Haagmans; Hans Clevers

doi:10.1038/s41467-021-25729-7

Nature Communications (Sep 2021)

A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

Joep Beumer,
Maarten H. Geurts,
Mart M. Lamers,
Jens Puschhof,
Jingshu Zhang,
Jelte van der Vaart,
Anna Z. Mykytyn,
Tim I. Breugem,
Samra Riesebosch,
Debby Schipper,
Petra B. van den Doel,
Wim de Lau,
Cayetano Pleguezuelos-Manzano,
Georg Busslinger,
Bart L. Haagmans,
Hans Clevers

Affiliations

Joep Beumer: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Maarten H. Geurts: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Mart M. Lamers: Viroscience Department, Erasmus Medical Center
Jens Puschhof: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Jingshu Zhang: Viroscience Department, Erasmus Medical Center
Jelte van der Vaart: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Anna Z. Mykytyn: Viroscience Department, Erasmus Medical Center
Tim I. Breugem: Viroscience Department, Erasmus Medical Center
Samra Riesebosch: Viroscience Department, Erasmus Medical Center
Debby Schipper: Viroscience Department, Erasmus Medical Center
Petra B. van den Doel: Viroscience Department, Erasmus Medical Center
Wim de Lau: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Cayetano Pleguezuelos-Manzano: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Georg Busslinger: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Bart L. Haagmans: Viroscience Department, Erasmus Medical Center
Hans Clevers: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center

DOI: https://doi.org/10.1038/s41467-021-25729-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology and identify the cell surface protease TMPRSS2 as a potential therapeutic target.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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