Nature Communications (Jan 2023)
The rapid and highly parallel identification of antibodies with defined biological activities by SLISY
- Steve Lu,
- Austin K. Mattox,
- P. Aitana Azurmendi,
- Ilias Christodoulou,
- Katharine M. Wright,
- Maria Popoli,
- Zan Chen,
- Surojit Sur,
- Yana Li,
- Challice L. Bonifant,
- Chetan Bettegowda,
- Nickolas Papadopoulos,
- Shibin Zhou,
- Sandra B. Gabelli,
- Bert Vogelstein,
- Kenneth W. Kinzler
Affiliations
- Steve Lu
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Austin K. Mattox
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- P. Aitana Azurmendi
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Ilias Christodoulou
- Department of Oncology, Johns Hopkins University School of Medicine
- Katharine M. Wright
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Maria Popoli
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Zan Chen
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Surojit Sur
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Yana Li
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Challice L. Bonifant
- Department of Oncology, Johns Hopkins University School of Medicine
- Chetan Bettegowda
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Nickolas Papadopoulos
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Shibin Zhou
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Sandra B. Gabelli
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Bert Vogelstein
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Kenneth W. Kinzler
- Ludwig Center, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-022-35668-6
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 13
Abstract
The covid pandemic has highlighted the need for rapid antibody development. Here, authors develop an approach called SLISY, which uses NGS with phage display to simultaneously assess millions of clones to rapidly isolate specific antibodies against SARS-CoV-2 and its evolving variants.
