Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools

Alexey Bondar; Olga Rybakova; Josef Melcr; Jan Dohnálek; Petro Khoroshyy; Ondřej Ticháček; Štěpán Timr; Paul Miclea; Alina Sakhi; Vendula Marková; Josef Lazar

doi:10.1038/s42003-021-01694-1

Communications Biology (Feb 2021)

Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools

Alexey Bondar,
Olga Rybakova,
Josef Melcr,
Jan Dohnálek,
Petro Khoroshyy,
Ondřej Ticháček,
Štěpán Timr,
Paul Miclea,
Alina Sakhi,
Vendula Marková,
Josef Lazar

Affiliations

Alexey Bondar: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Olga Rybakova: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Josef Melcr: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Jan Dohnálek: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Petro Khoroshyy: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Ondřej Ticháček: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Štěpán Timr: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Paul Miclea: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Alina Sakhi: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Vendula Marková: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
Josef Lazar: Institute of Organic Chemistry and Biochemistry, Czech Academy of Science

DOI: https://doi.org/10.1038/s42003-021-01694-1
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 12

Abstract

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Expanding on their previous work, Bondar et al present open source software tools to reliably quantify linear dichroism and determine molecular orientations. They demonstrate the utility of the tools by imaging synthetic lipid vesicles and as well as fluorescently labelled proteins in living cells

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal