A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Patrick M. Shih; Khanh Vuu; Nasim Mansoori; Leïla Ayad; Katherine B. Louie; Benjamin P. Bowen; Trent R. Northen; Dominique Loqué

doi:10.1038/ncomms13215

Nature Communications (Oct 2016)

A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Patrick M. Shih,
Khanh Vuu,
Nasim Mansoori,
Leïla Ayad,
Katherine B. Louie,
Benjamin P. Bowen,
Trent R. Northen,
Dominique Loqué

Affiliations

Patrick M. Shih: Joint BioEnergy Institute, Emery Station East
Khanh Vuu: Joint BioEnergy Institute, Emery Station East
Nasim Mansoori: Joint BioEnergy Institute, Emery Station East
Leïla Ayad: Joint BioEnergy Institute, Emery Station East
Katherine B. Louie: Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory
Benjamin P. Bowen: Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory
Trent R. Northen: Joint BioEnergy Institute, Emery Station East
Dominique Loqué: Joint BioEnergy Institute, Emery Station East

DOI: https://doi.org/10.1038/ncomms13215
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 8

Abstract

Read online

Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts, to efficiently assemble plant transformation vectors.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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