TMT-based proteomic analysis of Apocynum venetum under salt stress

Abstract

Abstract [Objective] Apocynum venetum L. is an important wild plant germplasm in China, which has important medicinal, ecological, and economic values. Although A . venetum is strong in salt tolerance, its wild population is gradually declining due to habitat destruction and increased soil salinization. Therefore, exploring the mechanism of salt tolerance in A . venetum is crucial for the protection and utilization of wild germplasm resources. [Methods] In the present study, a tandem mass tag (TMT)-based quantitative proteomics technology was used to conduct quantitative proteomic study of A . venetum under salt stress. Common differentially expressed proteins and core proteins were screened using Venn analysis and protein network interaction analysis methods. [Results] (1) Eleven common differentially expressed proteins were identified, which were mainly involved in protein-FAD linkage, negative regulation of transporter activity, negative regulation of ion transporter activity, negative regulation of anion transmembrane transport, negative regulation of anion channel activity, and thiazole metabolism. (2) Ribosomal proteins were the main proteins obtained by the interaction of differential protein networks at different stress times, among which the ribosomal protein L2-2 (TRINITY_DN13265_c0_g1_i1_9) was the core protein in differential proteins at 12 h and 24 h under stress. The results indicate that the ribosomal proteins play an important role in response to salt stress in A . venetum . [Conclusion] In the present study, the key proteins of A . venetum in response to salt stress were screened, and the molecular resources in response to salt stress in A . venetum were expanded, which provide theoretical bases for the discovery and utilization of natural resistant germplasms of A . venetum .

Keywords

• apocynum venetum
• proteomics
• salt stress
• molecular mechanism of salt tolerance