Respiratory Research (Sep 2009)

Expression profiling identifies genes involved in emphysema severity

  • Bowman Rayleen V,
  • Pavey Sandra J,
  • Larsen Jill E,
  • Francis Santiyagu,
  • Hayward Nicholas K,
  • Fong Kwun M,
  • Yang Ian A

DOI
https://doi.org/10.1186/1465-9921-10-81
Journal volume & issue
Vol. 10, no. 1
p. 81

Abstract

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Abstract Chronic obstructive pulmonary disease (COPD) is a major public health problem. The aim of this study was to identify genes involved in emphysema severity in COPD patients. Gene expression profiling was performed on total RNA extracted from non-tumor lung tissue from 30 smokers with emphysema. Class comparison analysis based on gas transfer measurement was performed to identify differentially expressed genes. Genes were then selected for technical validation by quantitative reverse transcriptase-PCR (qRT-PCR) if also represented on microarray platforms used in previously published emphysema studies. Genes technically validated advanced to tests of biological replication by qRT-PCR using an independent test set of 62 lung samples. Class comparison identified 98 differentially expressed genes (p p Gene expression profiling of lung from emphysema patients identified seven candidate genes associated with emphysema severity including COL6A3, SERPINF1, ZNHIT6, NEDD4, CDKN2A, NRN1 and GSTM3.