Co-Inoculation of Trichoderma asperellum with Bacillus subtilis to Promote Growth and Nutrient Absorption in Marandu Grass

Abstract

The authors have investigated the effects of different doses of mineral fertilizers combined with co-inoculation of Bacillus subtilis and Trichoderma asperellum to promote plant growth and use efficient nutrients in a plant Urochloa (Syn. Brachiaria) brizantha cv. Marandu. Individual experiments with doses of the nutrients nitrogen (N), phosphorus (P), and potassium (K) were carried out. The experiment was conducted in a completely randomized design, factorial 3 × 5. With the treatments: control noninoculation, inoculation with Bacillus subtilisUFRA-92, and coinoculation (MIX) with T. asperellum (UFRA-06, UFRA-09, UFRA-12, and UFRA-52) + B. subtilis x nutrient omissions (0%, 25%, 50%, 75%, and 100%). Each treatment had five replications. Biometric parameters, nutrient content, and nutrient use efficiency were evaluated. The results showed that the inoculants promoted growth in Marandu grass. The use of inoculants promoted growth and increased N, P, and K uptake by Marandu grass. Co-inoculation changed leaf area, shoot length, elongation rate, and leaf appearance for N and K and root dry mass for P. In addition, the co-inoculation combined with doses of 75% of N, 50% of P, and 50% of K increased the nutritional content of the leaves by 256% of N, 280% of P, and 29% of K and provided greater agronomic efficiency, with increments of 462% of N, 544% of P, and 177% of K, compared to the control treatment. We present the potential of co-inoculation of B. subtilis and T. asperellum to promote the growth of Urochloa under reduced fertilizer application. There was an improvement in plant growth and nutrient use efficiency.