A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide

Abstract

An amperometric biosensor for hydrogen peroxide (H2O2) was developed viaan easy and effective enzyme immobilization method with the “sandwich” configuration:ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basicelectrode. In order to prevent the loss of immobilized HRP under optimized conditions,the biosensor surface was cross-linked with glyoxal. Ferrocene was selected andimmobilized on the glassy carbon electrode surface as a mediator. The fabricationprocedure was systematically optimized to improve the biosensor performance. Thebiosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3.

Keywords

• horseradish peroxidase
• biosensor
• hydrogen peroxide
• chitosan