Enzyme-assisted ultrasonic extraction of total flavonoids and extraction polysaccharides in residue from Abelmoschus manihot (L)

Qiming Chu; Shengnan Xie; Hongling Wei; Xuchen Tian; Zhonghua Tang; Dewen Li; Ying Liu

Ultrasonics Sonochemistry (Mar 2024)

Enzyme-assisted ultrasonic extraction of total flavonoids and extraction polysaccharides in residue from Abelmoschus manihot (L)

Qiming Chu,
Shengnan Xie,
Hongling Wei,
Xuchen Tian,
Zhonghua Tang,
Dewen Li,
Ying Liu

Affiliations

Qiming Chu: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China
Shengnan Xie: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China
Hongling Wei: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China
Xuchen Tian: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China
Zhonghua Tang: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China
Dewen Li: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China; Corresponding authors.
Ying Liu: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry Based Active Substances, Harbin 150040, China; National Engineering Laboratory of BioResource EcoUtilization, Harbin 150040, China; Corresponding authors.

Journal volume & issue: Vol. 104
p. 106815

Abstract

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Abelmoschus manihot (L) is a traditional chinese herb and the present study focused on its comprehensive development and utilization. Enzyme-assisted ultrasonic extraction (EUAE) was investigated for the extraction and qualitative and quantitative analysis of flavonoids from Abelmoschus manihot (L) using a combination of ultra-performance liquid chromatography-photodiode array (UPLC-PDA), polysaccharides was extracted from residues and compared with directly extracted from raw materials. The optimal yield of 3.46±0.012 % (w/w) was obtained when the weight ratio of cellulase to pectinase was 1:1, the enzyme concentration was 3 %, the pH was 6.0, the solvent was a mixture of 70 % ethanol (v/v) and 0.1 mol/L NaH2PO4 buffer solution, the ultrasonic power was 500 W, the extraction time was 40 min, and the temperature of the extraction was 50 °C. The individual concentrations of interested flavonoids (rutin, neochlorogenic acid, nochlorogenic acid, lsoquercitrin, quercitrin, gossypin, quercetin) were effectively increased with the using of EUAE, compared with ultrasonic extraction (UE) method. Polysaccharides were extracted from each residue, respectively, the Polysaccharides yield in residue from EUAE was higher than that from UE, and closed to the yield from direct extraction in raw materials. The above results shown that the experimental process had the potential to be environmentall, friendly, straightforward and efficient.

Published in Ultrasonics Sonochemistry

ISSN: 1350-4177 (Print); 1873-2828 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry; Science: Physics: Acoustics. Sound
Website: https://www.journals.elsevier.com/ultrasonics-sonochemistry

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