A vegetable oil blend administration mitigates the hyperglycemia-induced redox imbalance, renal histopathology, and function in diabetic nephropathy

Abstract

The study was designed to search the efficacy of the oil blend, composed of sesame, canola, walnut, and wheat germ oils against diabetic nephropathy (DN). In the present study, the notion that a vegetable oil blend (VOB) minimizes oxidative damage, reduces renal injury, and preserves renal architecture was tested. Male wistar rats received VOB (2 ml/ kg−1) for 56 days after diabetes induction (streptozotocin 55 mg kg−1). Biochemical evidences in serum and renal tissue had been used to evaluate the impact on kidney injury and supported with histopathological examination. A significant increase in blood glucose, blood urea nitrogen (BUN), N-acetyl-β-D-glucosaminidase (NAG), and proteinuria levels with a concomitant decrease in glomerular filtration rate (GFR) were noted in diabetic rats. Oxidative damage indices such as 8-hydroxy-2′-deoxyguanosine (8-OHdG), transforming growth factor-beta1 (TGF-β1), malonaldehyde (MDA) and glutathione (GSH) were found higher in diabetic group with diminished glutathione reductase (GR) and superoxide dismutase (SOD) antioxidant enzymes level. Histological examination also revealed significant alterations, including glomerulosclerosis and interstitial fibrosis in diabetic group. Administration of VOB significantly modulated abnormalities in markers of renal dysfunction and tubular damage. Further oxidative damage and renal histological changes were recovered in the treatment group. In conclusion, our findings strongly suggest that renoprotective effect of VOB on nephrotic damage is attributable to its potential antidiabetic and antioxidant capabilities. There is still work to be done on a longer-term investigation or a clinical trial.

Keywords

• Diabetic nephropathy
• Vegetable oil blend
• Oxidative stress
• Nephroprotective