Bio-Protocol (Apr 2016)

Strategies for Performing Dynamic Gene Perturbation Experiments in Flowers

  • Diarmuid Ó’Maoiléidigh,
  • Emmanuelle Graciet,
  • Frank Wellmer

DOI
https://doi.org/10.21769/BioProtoc.1774
Journal volume & issue
Vol. 6, no. 7

Abstract

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Dissecting the gene regulatory networks (GRNs) underlying developmental processes is a central goal in biology. The characterization of the GRNs underlying flower development has received considerable attention, however, novel approaches are required to reveal temporal and spatial aspects of these GRNs. Here, we provide an overview of the options available to perform dynamic gene perturbations to identify downstream response genes at specific stages of development in the flowers of Arabidopsis thaliana.