The effect of alginate concentration on the viability of probiotics (Bacillus subtilis) in synbiotic microcapsule beads after incubation at 70°C

Abstract

High temperatures in feed molding machines can cause a decline in probiotic populations in fish feed. Adding alginate in probiotic preparations is expected to wrap probiotics so that when added to the feed before pelleting, it does not experience a drastic decrease. This study investigated the decline in probiotic populations in fish feed caused by high temperatures in feed molding machines and evaluated the use of alginate in probiotic preparations to prevent drastic decreases in viability. The study aimed to determine the viability of Bacillus sp. in synbiotic microcapsule beads with varying levels of alginate concentration after exposure to 70°C. The research used four treatments and three replicates with a randomized complete design. The results showed that the alginate concentration affected the diameter of the beads of synbiotic microcapsules, but it did not significantly affect the viability of Bacillus sp. The viability of probiotics in alginate beads after exposure to 70°C was 5.182±0.527 - 5.720±0.072 log cfu/ml. Therefore, using alginate in probiotic preparations can be considered as a reference for adding probiotics to fish feed before pelleting to maintain the viability of the probiotics in high-temperature conditions.