Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

Luqing Zhao; Xin Deng; Ning Ding; Yanzhen Liu; Yun Ma; Jiake Li; Shuqing Wang; Xingyu Ji; Jinjuan Wang; Dianpeng Wang; Yanhui Li; Dan Dou; Xihan Zhu; Lixin Zhu; Shengsheng Zhang

iScience (Aug 2024)

Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

Luqing Zhao,
Xin Deng,
Ning Ding,
Yanzhen Liu,
Yun Ma,
Jiake Li,
Shuqing Wang,
Xingyu Ji,
Jinjuan Wang,
Dianpeng Wang,
Yanhui Li,
Dan Dou,
Xihan Zhu,
Lixin Zhu,
Shengsheng Zhang

Affiliations

Luqing Zhao: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Xin Deng: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Ning Ding: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Yanzhen Liu: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Yun Ma: Beijing University of Chinese Medicine, Beijing 100105, China
Jiake Li: Beijing University of Chinese Medicine, Beijing 100105, China
Shuqing Wang: Beijing University of Chinese Medicine, Beijing 100105, China
Xingyu Ji: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Jinjuan Wang: School of Mathematics and Statistics, Beijing Institute of Technology, Beijing 100081, China
Dianpeng Wang: School of Mathematics and Statistics, Beijing Institute of Technology, Beijing 100081, China
Yanhui Li: School of Mathematics and Statistics, Beijing Institute of Technology, Beijing 100081, China
Dan Dou: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Xihan Zhu: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
Lixin Zhu: Department of General Surgery (Colorectal Surgery), Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, Biomedical Innovation Center, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China; Corresponding author
Shengsheng Zhang: Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China; Corresponding author

Journal volume & issue: Vol. 27, no. 8
p. 110403

Abstract

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Summary: Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing Actinomyces and Akkermansia in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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