Biofilm reactor calibration for in vitro investigation of composite biodegradation

Abstract

Introduction: The majority of biodegradation studies of composite materials use simplified models of microbial biofilm despite the apparent diversity of the oral microbiota. The use of in vitro systems of “artificial mouth” design is a step towards clarifying the synergistic effect that microbial plaque and human saliva have on composite degradation. Aim: Establishment of functional parameters for in vitro reproduction of oral biofilms via biofilm reactor systems. Materials and methods: The CDC Biofilm Reactor system consists of eight polypropylene sticks. The rod cover and the retaining plates are mounted in a 1-dm glass cylinder with an outlet side opening. The laboratory bioreactor has a working volume of 340 ml. The device is equipped with a four-blade magnetic stirrer. The system also includes gauging appliances and executive mechanisms for controlling and adjusting the basic parameters of the process. Results: Determination of the operating volume of the reactor is performed prior to the experiment along with the time of reach and stabilization of the set temperature in the design which is 60 min at 120 rpm. A mathematical model is used to calculate the rate of delivery of growth medium - 11 millilitres per minute. The bioreactor is sterilized by 0.3% neomycin solution for 24 hours. Prior to the experiment the system is cleansed (via passage) with sterile water for 60 minutes. Conclusions: The pre-calibration of a bioreactor system allows specification and refinement of its working parameters, thus engaging for accurate reproduction of the environmental conditions in the oral cavity.