Correlation between serum level of PARP1 and vascular calcification in hemodialysis patients

Abstract

Objective To explore the correlation between serum level of poly(ADP-ribose) polymerase 1 (PARP1) and vascular calcification in hemodialysis (HD) patients. Methods From January 2020 to December 2021, 168 hospitalized HD patients were selected for retrospective analysis. According to the degree of vascular calcification as evaluated by coronary artery calcification score, they were assigned into four groups of non-calcification (n=45), mild calcification (n=26), moderate calcification (n=53) and severe calcification (n=44). Enzyme-linked immunosorbent assay (ELISA) was utilized for detecting serum level of PARP1. Spearman’s method was employed for examining the correlation between serum level of PARP1 and vascular calcification score. And the correlation between serum level of PARP1 and the levels of albumin (Alb), hemoglobin (Hb), calcium (Ca2+), phosphorus (P3−) and parathyroid hormone (PTH) was analyzed by Pearson’s method. Logistic regression analysis was performed for the influencing factors of the occurrence of vascular calcification. Diagnostic value of serum level of PARP1 for vascular calcification was analyzed by receiver operating characteristic (ROC) curve. Results Serum level of PARP1 without calcification was (4.05±1.18)μg/L. As compared with non-calcification group, serum level of PARP1 was obviously higher in mild/moderate/severe calcification group (P<0.05). The greater degree of vascular calcification, the higher value of PARP1 and calcification score (P<0.05); serum level of PARP1 was correlated positively with calcification score, the levels of Ca2+, P3−, PTH and hs-CRP (calcification score: r=0.662, P<0.05; Ca2+: r=0.547, P<0.05; P3−: r=0.421, P<0.05; PTH: r=0.652, P<0.05; hs-CRP: r=0.640, P<0.05) were correlated negatively with the levels of Alb and Hb (Alb: r=−0.600, P<0.05; Hb: r=−0.616, P<0.05); Logistic regression analysis indicated that age, dialysis age, Ca2+, P3−, PTH, hs-CRP and PARP1 were the risk factors for vascular calcification (P<0.05) while Alb and Hb served as the protective factors for vascular calcification (P<0.05). Area under the ROC curve (AUC) of vascular calcification diagnosed by serum level of PARP1 was 0.936 with a cut-off value of 4.39 μg/L. Conclusions Serum level of PARP1 rises with worsening vascular calcification. It may better diagnose vascular calcification in HD patients.

Keywords

• hemodialysis
• vascular calcification
• poly(adp-ribose) polymerase