iScience (Sep 2023)
Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines
- Julie Van Coillie,
- Tamas Pongracz,
- Tonći Šuštić,
- Wenjun Wang,
- Jan Nouta,
- Mathieu Le Gars,
- Sofie Keijzer,
- Federica Linty,
- Olvi Cristianawati,
- Jim B.D. Keijser,
- Remco Visser,
- Lonneke A. van Vught,
- Marleen A. Slim,
- Niels van Mourik,
- Merel J. Smit,
- Adam Sander,
- David E. Schmidt,
- Maurice Steenhuis,
- Theo Rispens,
- Morten A. Nielsen,
- Benjamin G. Mordmüller,
- Alexander P.J. Vlaar,
- C. Ellen van der Schoot,
- Ramon Roozendaal,
- Manfred Wuhrer,
- Gestur Vidarsson,
- Brent Appelman,
- Diederik van de Beek,
- Marije K. Bomers,
- Justin de Brabander,
- Matthijs C. Brouwer,
- David T.P. Buis,
- Nora Chekrouni,
- Marit J. van Gils,
- Menno D. de Jong,
- Ayesha H.A. Lavell,
- Niels van Mourik,
- Sabine E. Olie,
- Edgar J.G. Peters,
- Tom D.Y. Reijnders,
- Michiel Schinkel,
- Alex R. Schuurman,
- Jonne J. Sikkens,
- Marleen A. Slim,
- Yvo M. Smulders,
- Alexander P.J. Vlaar,
- Lonneke A. van Vught,
- Joost W. Wiersinga,
- Antinori Spinello,
- Cinzia Bassoli,
- Giovanna Bestetti,
- Mario Corbellino,
- Alice Covizzi,
- Angelica Lupo,
- Laura Milazzo,
- Marco Schiuma,
- Alessandro Torre,
- Willem A. de Jongh,
- Ali Salanti,
- Thor G. Theander,
- Matthew B.B. McCall,
- Meral Esen
Affiliations
- Julie Van Coillie
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands; Department of Biomolecular Mass Spectrometry and Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
- Tamas Pongracz
- Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
- Tonći Šuštić
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands; Department of Biomolecular Mass Spectrometry and Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
- Wenjun Wang
- Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
- Jan Nouta
- Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
- Mathieu Le Gars
- Janssen Vaccines & Prevention B.V., Leiden, the Netherlands
- Sofie Keijzer
- Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands
- Federica Linty
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands; Department of Biomolecular Mass Spectrometry and Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
- Olvi Cristianawati
- Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands
- Jim B.D. Keijser
- Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands
- Remco Visser
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands; Department of Biomolecular Mass Spectrometry and Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
- Lonneke A. van Vught
- Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity Institute, Amsterdam, the Netherlands; Department of Intensive Care, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands
- Marleen A. Slim
- Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity Institute, Amsterdam, the Netherlands; Department of Intensive Care, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands
- Niels van Mourik
- Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity Institute, Amsterdam, the Netherlands; Department of Intensive Care, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands
- Merel J. Smit
- Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, the Netherlands
- Adam Sander
- Centre for Medical Parasitology, Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; AdaptVac Aps, Copenhagen, Denmark
- David E. Schmidt
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands
- Maurice Steenhuis
- Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands
- Theo Rispens
- Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands
- Morten A. Nielsen
- Centre for Medical Parasitology, Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
- Benjamin G. Mordmüller
- Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, the Netherlands
- Alexander P.J. Vlaar
- Department of Intensive Care, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands; Laboratory of Experimental Intensive Care and Anaesthesiology, L.E.I.C.A., Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands
- C. Ellen van der Schoot
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands
- Ramon Roozendaal
- Janssen Vaccines & Prevention B.V., Leiden, the Netherlands
- Manfred Wuhrer
- Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
- Gestur Vidarsson
- Department of Experimental Immunohematology, Sanquin Research, Amsterdam, the Netherlands; Department of Biomolecular Mass Spectrometry and Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands; Corresponding author
- Brent Appelman
- Diederik van de Beek
- Marije K. Bomers
- Justin de Brabander
- Matthijs C. Brouwer
- David T.P. Buis
- Nora Chekrouni
- Marit J. van Gils
- Menno D. de Jong
- Ayesha H.A. Lavell
- Niels van Mourik
- Sabine E. Olie
- Edgar J.G. Peters
- Tom D.Y. Reijnders
- Michiel Schinkel
- Alex R. Schuurman
- Jonne J. Sikkens
- Marleen A. Slim
- Yvo M. Smulders
- Alexander P.J. Vlaar
- Lonneke A. van Vught
- Joost W. Wiersinga
- Antinori Spinello
- Cinzia Bassoli
- Giovanna Bestetti
- Mario Corbellino
- Alice Covizzi
- Angelica Lupo
- Laura Milazzo
- Marco Schiuma
- Alessandro Torre
- Willem A. de Jongh
- Ali Salanti
- Thor G. Theander
- Matthew B.B. McCall
- Meral Esen
- Journal volume & issue
-
Vol. 26,
no. 9
p. 107619
Abstract
Summary: IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.
