Identification and Full-Length Sequence Analysis of a Novel Recombinant Goat Astrovirus Genotype in Guangxi, China

Yulu Zhou; Pengju Xu; Yanhua Huang; Jie Wang; Chang Cui; Yanglin Wang; Yuhang Luo; Xiaoling Wang; Jiang Xie; Fengmei Li; Zuzhang Wei; Ying Chen; Kang Ouyang; Yifeng Qin; Yan Pan; Weijian Huang

doi:10.3390/v16081213

Viruses (Jul 2024)

Identification and Full-Length Sequence Analysis of a Novel Recombinant Goat Astrovirus Genotype in Guangxi, China

Yulu Zhou,
Pengju Xu,
Yanhua Huang,
Jie Wang,
Chang Cui,
Yanglin Wang,
Yuhang Luo,
Xiaoling Wang,
Jiang Xie,
Fengmei Li,
Zuzhang Wei,
Ying Chen,
Kang Ouyang,
Yifeng Qin,
Yan Pan,
Weijian Huang

Affiliations

Yulu Zhou: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Pengju Xu: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yanhua Huang: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Jie Wang: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Chang Cui: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yanglin Wang: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yuhang Luo: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Xiaoling Wang: Laboratory for the Prevention and Control of Bovine and Goat Diseases, College of Animal Science and Technology, Guangxi Vocational University of Agriculture, Nanning 530007, China
Jiang Xie: Laboratory for the Prevention and Control of Bovine and Goat Diseases, College of Animal Science and Technology, Guangxi Vocational University of Agriculture, Nanning 530007, China
Fengmei Li: Laboratory for the Prevention and Control of Bovine and Goat Diseases, College of Animal Science and Technology, Guangxi Vocational University of Agriculture, Nanning 530007, China
Zuzhang Wei: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Ying Chen: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Kang Ouyang: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yifeng Qin: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yan Pan: Laboratory for the Prevention and Control of Bovine and Goat Diseases, College of Animal Science and Technology, Guangxi Vocational University of Agriculture, Nanning 530007, China
Weijian Huang: Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China

DOI: https://doi.org/10.3390/v16081213
Journal volume & issue: Vol. 16, no. 8
p. 1213

Abstract

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Astroviruses are single-stranded, positive-sense RNA viruses capable of infecting humans as well as a wide range of mammalian and avian species, with a length of approximately 6.6–7.7 kb. In this study, 139 goat fecal samples collected from the Guangxi province were used for the RT-PCR detection, and two of these were positive for goat astrovirus, with a positivity rate of 1.44% (2/139). The complete genome sequence of an astrovirus strain and the partial genome sequence of a strain astrovirus, named GX WZ 2023 and GX HC 2023, were amplified and sequenced, and their sequence lengths were 6284 nt and 6213 nt, respectively. Among them, the capsid protein of goat astrovirus GX HC 2023 showed the highest amino acid identity of 95.9% with ovine astrovirus GX, which belonged to the MAstV-2 genotype. However, the closest relative of the GX WZ 2023 strain was found to be the caprine astrovirus Sichuan, with a nucleotide sequence identity of 76.8%. The ORF1ab nonstructural protein of this strain showed the highest amino acid identities of 89.2 and 95.8% with the ovine astrovirus S5.1 and caprine astrovirus G5.1 strains, respectively. However, its ORF2 capsid protein has 68.4% amino acid identity with the bovine astrovirus (BAstV) 16 2021 CHN strain and only 21.9–64% amino acid identity with all available strains of goat astrovirus. The GX WZ 2023 strain was recombined with the Chinese (BAstV 16 2021 CHN) and Japanese bovine strains (BAstV JPN 2015) in the ORF2 region. Therefore, the goat astrovirus GX WZ 2023 is proposed as a new member of the family goat astroviridae based on the species classification criteria of the International Committee on Taxonomy of Viruses. These findings enhance our understanding of the prevalence and genetic evolution of goat astrovirus and provide a scientific basis for future studies of these viruses in other animals.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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