Single cell sequencing in the study of hematopoietic system: application and perspects

Abstract

Single cell sequencing (SCS) is a technique for precise analysis of cells at an individual level, which provides a new perspective on the identification of individual cell types, functions, states and interactions with other cells, and greatly deepens the understanding of cellular heterogeneity and genetic and molecular functional diversity. With the continuous optimization of various technical aspects, SCS technology has been widely used in many research areas, and the study of the hematopoietic system is an example of its application. SCS has not only assisted in the construction of relevant hematopoietic cell profiles under physiological and pathophysiological conditions in basic research, but also explored possible pathways of hematopoietic cell development and differentiation in clinical practice. Moreover, in the process of clinical diagnosis and treatment, it can effectively promote the deciphering of mechanisms of disease occurrence, progression and prognosis, and facilitate the exploration of potential factors of phenomena such as significant differences in individual outcomes and ease of relapse. Therefore, this paper reviews the technical overview and development trend of SCS and its application in the hematopoietic system, with a view to providing references for subsequent positive and reasonable application of SCS.

Keywords

• single cell sequencing
• hematopoietic system
• development and differentiation
• clinical treatment