Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery

Wei Qin; Samuel A. Myers; Dominique K. Carey; Steven A. Carr; Alice Y. Ting

doi:10.1038/s41467-021-25259-2

Nature Communications (Aug 2021)

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery

Wei Qin,
Samuel A. Myers,
Dominique K. Carey,
Steven A. Carr,
Alice Y. Ting

Affiliations

Wei Qin: Departments of Biology, Genetics, and Chemistry, Stanford University
Samuel A. Myers: The Broad Institute of MIT and Harvard
Dominique K. Carey: The Broad Institute of MIT and Harvard
Steven A. Carr: The Broad Institute of MIT and Harvard
Alice Y. Ting: Departments of Biology, Genetics, and Chemistry, Stanford University

DOI: https://doi.org/10.1038/s41467-021-25259-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

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Proximity labeling is used to map and discover proteins in specific subcellular compartments. Here the authors combine APEX-mediated proximity labeling with organic-aqueous phase separation to identify nuclear, nucleolar, and outer mitochondrial membrane RNA binding proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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