COMPLETE GENOME SEQUENCE OF THE PREDATORY MITE NEOSEIULUS CALIFORNICUS (MESOSTIGMATA, PHYTOSEIIDAE) FROM LABORATORY STRAIN (BIODEFENCE)

Abstract

Neoseiulus californicus is an effective predatory mite for spider mites biocontrol. We sequenced and assembled the genomes of two isofemale lines of N. californicus: BioDefence and BioDefence2, derived from a biocontrol mite population, using the Oxford Nanopore long-read sequencing method. A total of 3507265 reads were obtained for the genome of the BioDefence line and 1769217 reads for the BioDefence2 line. Canu v.2.3 software was used for contig assembly. The genome of the BioDefence line was assembled as 419 contigs of 191.4 Mb in length, with a G+C content of 50.33%. The N50 is 13147 and the average coverage is 26.798. The annotation identified – 12253 genes, including 11607 protein-coding genes, 28 microsatellites, 9 families of small RNA genes, 2 families of LTR retrotransposons, 3 families of DNA transposons, 12 rRNA genes and 634 tRNA genes. The genome of line BioDefence2 was obtained using a similar procedure. Based on the contigs obtained, we assembled two new complete mitochondrial genomes of N. californicus from the BioDefence and BioDefence2 lines. We identified hypervariable regions of the genome within the mitochondrial control region that show inter-lineage variability. This study adds a new complete genome of N. californicus assembled at the contig level to the two other complete N. californicus genomes registered in GenBank and assembled at the contig level.

Keywords

• amblyseiinae
• oxford nanopore minion
• длинные прочтения
• сборка генома
• amblyseiinae
• oxford nanopore minion
• long reads
• genome assembly