Identification of the potential mechanisms of Huzhang Tongfeng Granules against acute gouty arthritis through network pharmacology and in vivo analysis

Mi Zhou; Ying Zhang; Zhan Zhang; Xiaojie Ding; Yue Luo; Xiaoya Fei; Jiankun Song; Dan Yang; Siting Chen; Bin Li; Le Kuai

Pharmacological Research - Modern Chinese Medicine (Sep 2022)

Identification of the potential mechanisms of Huzhang Tongfeng Granules against acute gouty arthritis through network pharmacology and in vivo analysis

Mi Zhou,
Ying Zhang,
Zhan Zhang,
Xiaojie Ding,
Yue Luo,
Xiaoya Fei,
Jiankun Song,
Dan Yang,
Siting Chen,
Bin Li,
Le Kuai

Affiliations

Mi Zhou: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Ying Zhang: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Zhan Zhang: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Xiaojie Ding: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Yue Luo: Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China
Xiaoya Fei: Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China
Jiankun Song: Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China
Dan Yang: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Siting Chen: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
Bin Li: Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors at: Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China.
Le Kuai: Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China; Corresponding authors at: Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China.

Journal volume & issue: Vol. 4
p. 100131

Abstract

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Objective: To identify the potential mechanism of Huzhang Tongfeng Granules (HTG) for the treatment of acute gouty arthritis (AGA). Methods: First of all, we used network pharmacology to build a network of components-targets of HTG. Secondly, the ClusterONE algorithm was applied to construct a modular network as well as discover hub genes and kernel pathways. Lastly, the MSU crystals-induced AGA mice model was used to verify the hub genes and kernel pathways of HTG. Results: HTG down-regulated the expressions of ADRB3, PIK3CA, FYN, PIK3R1, CASR, PLCG1, SYK, EGFR, and up-regulated MC1R, MC5R. Moreover, according to the kernel clusters enrichment analysis, HTG down-regulated NF-κB and P13K-Akt signaling pathways to ameliorate the AGA mice model. Conclusion: In summary, the potential targets and kernel pathways were proved in this study, supporting the clinical application of HTG for the treatment of AGA.

Published in Pharmacological Research - Modern Chinese Medicine

ISSN: 2667-1425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Other systems of medicine; Medicine: Therapeutics. Pharmacology
Website: https://www.sciencedirect.com/journal/pharmacological-research-modern-chinese-medicine

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