IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China

Siyi Wang; Juan Zhao; Ning Liu; Fang Yang; Yiming Zhong; Xiumei Gu; Zijuan Jian; Qun Yan; Qingxia Liu; Hongling Li; Yanming Li; Jing Liu; Hui Li; Liang Chen; Wenen Liu

doi:10.1128/mSphere.00407-20

mSphere (Aug 2020)

IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China

Siyi Wang,
Juan Zhao,
Ning Liu,
Fang Yang,
Yiming Zhong,
Xiumei Gu,
Zijuan Jian,
Qun Yan,
Qingxia Liu,
Hongling Li,
Yanming Li,
Jing Liu,
Hui Li,
Liang Chen,
Wenen Liu

Affiliations

Siyi Wang: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Juan Zhao: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Ning Liu: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Fang Yang: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Yiming Zhong: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Xiumei Gu: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Zijuan Jian: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Qun Yan: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Qingxia Liu: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Hongling Li: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Yanming Li: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China
Jing Liu: Molecular Biology Research Center and Hunan Province Key Laboratory of Basic and Applied Hematology, School of Life Sciences, Central South University, Changsha, China
Hui Li: Molecular Biology Research Center and Hunan Province Key Laboratory of Basic and Applied Hematology, School of Life Sciences, Central South University, Changsha, China
Liang Chen: Center for Discovery and Innovation, Hackensack-Meridian Health, Nutley, New Jersey, USA
Wenen Liu: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, China

DOI: https://doi.org/10.1128/mSphere.00407-20
Journal volume & issue: Vol. 5, no. 4

Abstract

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ABSTRACT An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among carbapenem-resistant Klebsiella pneumoniae isolates from a tertiary care hospital in central China. A total of 14 IMP-38-producing ST307 K. pneumoniae strains were identified from 2013 to 2016, with 13 strains isolated from patients with neonatal sepsis in the neonatal ward. PacBio and Illumina whole-genome sequencing analysis performed on a representative IMP-38-producing K. pneumoniae strain, WCGKP294, showed that it contained a circular chromosome and two plasmids. Carbapenemase gene blaIMP-38 is colocated with blaCTX-M-3 in transposon Tn6382 on an IncHI5 plasmid (pWCGKP294-2). WCGKP294 harbors another IncFIB plasmid, pWCGKP294-1, carrying three copies of tandem-repeated IS26-blaSHV-2A-deoR-ygbJ-ygbK-fucA-IS26 composite transposon elements. Phylogenetic analysis placed WCGKP294 in the global ST307 cluster, distant from the U.S. (Texas) and South Africa clusters. Nevertheless, WCGKP294 does not contain the chromosomal fluoroquinolone resistance-associated mutations and IncFIIK/IncFIBK plasmid-associated blaCTX-M-15 gene that are frequently found in other global ST307 strains. IMPORTANCE We described the genome and resistome characterization of a carbapenem-resistant Klebsiella pneumoniae ST307 strain carrying blaIMP-38 in China. This report highlights that the high-risk ST307 clone continues to acquire different antimicrobial resistance genes, posing significant challenges to clinical practice, and should be closely monitored.

Published in mSphere

ISSN: 2379-5042 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msphere

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