High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening

Rodrigo A. Gier; Krista A. Budinich; Niklaus H. Evitt; Zhendong Cao; Elizabeth S. Freilich; Qingzhou Chen; Jun Qi; Yemin Lan; Rahul M. Kohli; Junwei Shi

doi:10.1038/s41467-020-17209-1

Nature Communications (Jul 2020)

High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening

Rodrigo A. Gier,
Krista A. Budinich,
Niklaus H. Evitt,
Zhendong Cao,
Elizabeth S. Freilich,
Qingzhou Chen,
Jun Qi,
Yemin Lan,
Rahul M. Kohli,
Junwei Shi

Affiliations

Rodrigo A. Gier: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Krista A. Budinich: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Niklaus H. Evitt: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Zhendong Cao: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Elizabeth S. Freilich: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Qingzhou Chen: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania
Jun Qi: Department of Cancer Biology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School
Yemin Lan: Epigenetics Institute, Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania
Rahul M. Kohli: Epigenetics Institute, Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania
Junwei Shi: Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania

DOI: https://doi.org/10.1038/s41467-020-17209-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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