In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture

Shannon G. Klein; Samhan M. Alsolami; Silvia Arossa; Gerardo Ramos-Mandujano; Anieka J. Parry; Alexandra Steckbauer; Carlos M. Duarte; Mo Li

doi:10.1038/s42003-022-03065-w

Communications Biology (Feb 2022)

In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture

Shannon G. Klein,
Samhan M. Alsolami,
Silvia Arossa,
Gerardo Ramos-Mandujano,
Anieka J. Parry,
Alexandra Steckbauer,
Carlos M. Duarte,
Mo Li

Affiliations

Shannon G. Klein: Red Sea Research Center (RSRC) and Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology
Samhan M. Alsolami: Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology
Silvia Arossa: Red Sea Research Center (RSRC) and Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology
Gerardo Ramos-Mandujano: Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology
Anieka J. Parry: Red Sea Research Center (RSRC) and Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology
Alexandra Steckbauer: Red Sea Research Center (RSRC) and Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology
Carlos M. Duarte: Red Sea Research Center (RSRC) and Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology
Mo Li: Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology

DOI: https://doi.org/10.1038/s42003-022-03065-w
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

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Luminescence-based gas measurements on human cell cultures show deviations from physiological conditions, highlighting the need for a careful consideration of experimental results obtained with these standard cell lines.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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