A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice

Jiali Wu; Meiyan Wang; Xueping Yang; Chengwei Yi; Jian Jiang; Yuanhuan Yu; Haifeng Ye

doi:10.1038/s41467-020-17530-9

Nature Communications (Jul 2020)

A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice

Jiali Wu,
Meiyan Wang,
Xueping Yang,
Chengwei Yi,
Jian Jiang,
Yuanhuan Yu,
Haifeng Ye

Affiliations

Jiali Wu: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Meiyan Wang: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Xueping Yang: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Chengwei Yi: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Jian Jiang: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Yuanhuan Yu: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
Haifeng Ye: Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University

DOI: https://doi.org/10.1038/s41467-020-17530-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Current light-inducible Cre-loxP systems have minimal capacity for deep tissue penetration. Here, the authors present a far-red light-induced split Cre-loxP system for in vivo genome engineering.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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