Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

Shiran Bar; Dan Vershkov; Gal Keshet; Elyad Lezmi; Naama Meller; Atilgan Yilmaz; Ofra Yanuka; Malka Nissim-Rafinia; Eran Meshorer; Talia Eldar-Geva; Nissim Benvenisty

doi:10.1038/s41467-021-26949-7

Nature Communications (Nov 2021)

Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

Shiran Bar,
Dan Vershkov,
Gal Keshet,
Elyad Lezmi,
Naama Meller,
Atilgan Yilmaz,
Ofra Yanuka,
Malka Nissim-Rafinia,
Eran Meshorer,
Talia Eldar-Geva,
Nissim Benvenisty

Affiliations

Shiran Bar: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Dan Vershkov: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Gal Keshet: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Elyad Lezmi: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Naama Meller: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Atilgan Yilmaz: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Ofra Yanuka: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Malka Nissim-Rafinia: Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Eran Meshorer: Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram
Talia Eldar-Geva: IVF Unit, Division of Obstetrics and Gynecology, Shaare Zedek Medical Center
Nissim Benvenisty: The Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram

DOI: https://doi.org/10.1038/s41467-021-26949-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Genetic imprinting ensures monoallelic gene expression critical for normal embryonic development. Here the authors take advantage of human haploid parthenogenic embryonic stem cells lacking paternal alleles to identify, by genome-wide screening, factors involved in the regulation of imprinted genes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal