Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics

Brandon Hadland; Barbara Varnum-Finney; Stacey Dozono; Tessa Dignum; Cynthia Nourigat-McKay; Adam M. Heck; Takashi Ishida; Dana L. Jackson; Tomer Itkin; Jason M. Butler; Shahin Rafii; Cole Trapnell; Irwin D. Bernstein

doi:10.1038/s41467-022-28781-z

Nature Communications (Mar 2022)

Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics

Brandon Hadland,
Barbara Varnum-Finney,
Stacey Dozono,
Tessa Dignum,
Cynthia Nourigat-McKay,
Adam M. Heck,
Takashi Ishida,
Dana L. Jackson,
Tomer Itkin,
Jason M. Butler,
Shahin Rafii,
Cole Trapnell,
Irwin D. Bernstein

Affiliations

Brandon Hadland: Clinical Research Division, Fred Hutchinson Cancer Research Center
Barbara Varnum-Finney: Clinical Research Division, Fred Hutchinson Cancer Research Center
Stacey Dozono: Clinical Research Division, Fred Hutchinson Cancer Research Center
Tessa Dignum: Clinical Research Division, Fred Hutchinson Cancer Research Center
Cynthia Nourigat-McKay: Clinical Research Division, Fred Hutchinson Cancer Research Center
Adam M. Heck: Clinical Research Division, Fred Hutchinson Cancer Research Center
Takashi Ishida: Clinical Research Division, Fred Hutchinson Cancer Research Center
Dana L. Jackson: Department of Genome Sciences, University of Washington School of Medicine
Tomer Itkin: Department of Genetic Medicine, Ansary Stem Cell Institute, Howard Hughes Medical Institute, Weill Cornell Medical College
Jason M. Butler: Center for Discovery and Innovation, Hackensack University Medical Center
Shahin Rafii: Department of Genetic Medicine, Ansary Stem Cell Institute, Howard Hughes Medical Institute, Weill Cornell Medical College
Cole Trapnell: Department of Genome Sciences, University of Washington School of Medicine
Irwin D. Bernstein: Clinical Research Division, Fred Hutchinson Cancer Research Center

DOI: https://doi.org/10.1038/s41467-022-28781-z
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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Here, the authors use single cell RNA-sequencing to generate an atlas of signaling interactions regulating embryonic hematopoietic stem cell (HSC) development and apply this knowledge to engineer a niche sufficient to support HSC maturation in vitro.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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