On application of emulsified probiotic preparation of Bacillus subtilis

Abstract

The use of a new form of the probiotic preparation of Bacillus subtilis consisting in emulsion of bacteria in palmitic acid has been studied. When evaluating the effectiveness of probiotics, it was assumed that the content of bacterial DNA in the feces is proportional to their content in the intestine. Analysis of the number of bacterial DNA was carried out by real-time PCR. The results showed a positive effect from the use of emulsified bacteria. It was observed in an increase in the amount of B. subtilis in the intestine and, as a consequence, their DNA in the feces of rats.