Transcriptomic analysis reveals the defense mechanisms of citrus infested with Diaphorina citri

Abstract

Huanglongbing (HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world's most important fruit crops. The phloem-feeding Asian citrus psyllid (ACP), Diaphorina citri, is the main pest at the new shoot stage and is the only natural vector of HLB pathogenic bacteria. Little is known about how plants perceive and defend themselves from this destructive pest. Here, we characterized changes in the expression of various genes in citrus plants that were continuously infested by D. citri for different durations (12, 24, and 48 h). A total of 5 219 differentially expressed genes (DEGs) and 643 common DEGs were identified across all time points. Several pathways related to defense were activated, such as peroxisome, alpha-linolenic acid metabolism, and phenylpropanoid and terpenoid biosynthesis, and some pathways related to growth and signal transduction were suppressed in response to D. citri infestation. The expression of genes including kinases (CML44, CIPK6, and XTH6), phytohormones (SAMT, LOX6, and NPR3), transcription factors (bHLH162, WRKY70, and WRKY40), and secondary metabolite synthesis-related genes (PAL, 4CL2, UGT74B1 and CYP82G1) was significantly altered in response to D. citri infestation. The findings of this study greatly enhance our understanding of the mechanisms underlying the defense response of citrus plants to D. citri infestation at the molecular level. Functional characterization of the candidate defense-related genes identified in this study will aid the molecular breeding of insect-resistant citrus varieties.

Keywords

• Asian citrus psyllid
• Citrus
• Diaphorina citri
• Huanglongbing
• Infestation
• RNA-seq