BMB Reports (Mar 2012)

Mxi1 influences cyst formation in three-dimensional cell culture

  • Yeon Joo Yook,
  • Kyung Hyun Yoo,
  • Seon Ah Song,
  • Min Ji Seo,
  • Je Yeong Ko,
  • Bo Hye Kim,
  • Eun Ji Lee,
  • EunSun Chang,
  • Yu Mi Woo,
  • Jong Hoon Park

DOI
https://doi.org/10.5483/BMBRep.2012.45.3.189
Journal volume & issue
Vol. 45, no. 3
pp. 189 – 193

Abstract

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Cyst formation is a major characteristic of ADPKD and iscaused by the abnormal proliferation of epithelial cells. Renalcyst formation disrupts renal function and induces diversecomplications. The mechanism of cyst formation is unclear.mIMCD-3 cells were established to develop simple epithelialcell cysts in 3-D culture. We confirmed previously that Mxi1plays a role in cyst formation in Mxi1-deficient mice. Cysts inMxi1 transfectanted cells were showed by collagen or mebiolgels in 3-D cell culture system. Causative genes of ADPKDwere measured by q RT-PCR. Herein, Mxi1 transfectants rarelyformed a simple epithelial cyst and induced cell death.Overexpression of Mxi1 resulted in a decrease in the PKD1,PKD2 and c-myc mRNA relating to the pathway of cystformation. These data indicate that Mxi1 influences cystformation of mIMCD-3 cells in 3-D culture and that Mxi1 maycontrol the mechanism of renal cyst formation. [BMB reports2012; 45(3): 189-193]

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