BMC Veterinary Research (Jul 2024)
Mortality in sea lions is associated with the introduction of the H5N1 clade 2.3.4.4b virus in Brazil October 2023: whole genome sequencing and phylogenetic analysis
- Andreina de Carvalho Araujo,
- Andrew Yong Cho,
- Laura Morais Nascimento Silva,
- Thais Camilo Corrêa,
- Gabriela Cristini de Souza,
- Adriana Silva Albuquerque,
- Eduardo Macagnan,
- Cristiane K. M. Kolesnikvoas,
- Rafael Meurer,
- Jenyffer Vierheller Vieira,
- Giulia Gaglianone Lemos,
- André Silva Barreto,
- Jeferson Luis Dick,
- Karina Rejane Groch,
- Pedro Volkmer de Castilho,
- Deyvid Amgarten,
- Fernanda Malta,
- Michael Miller,
- Erick G. Dorlass,
- Soledad Palameta,
- Sun-Hak Lee,
- Clarice Weis Arns,
- Edison L. Durigon,
- João Renato R. Pinho,
- Dong-Hun Lee,
- Helena Lage Ferreira
Affiliations
- Andreina de Carvalho Araujo
- Department of Veterinary Medicine, FZEA- USP, University of São Paulo
- Andrew Yong Cho
- Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University
- Laura Morais Nascimento Silva
- Graduate Program in Experimental Epidemiology Applied to Zoonoses, Veterinary Medicine and Animal Science School, University of São Paulo
- Thais Camilo Corrêa
- Department of Veterinary Medicine, FZEA- USP, University of São Paulo
- Gabriela Cristini de Souza
- Zoology Laboratory, Stabilization Unit, Laguna, UDESC
- Adriana Silva Albuquerque
- Zoology Laboratory, Stabilization Unit, Laguna, UDESC
- Eduardo Macagnan
- Zoology Laboratory, Stabilization Unit, Laguna, UDESC
- Cristiane K. M. Kolesnikvoas
- R3 Animal Association
- Rafael Meurer
- R3 Animal Association
- Jenyffer Vierheller Vieira
- Marine Tetrapod Ecology and Conservation Laboratory, Marine Animal Stabilization Unit, São Francisco do Sul, University of the Joinville Region, UNIVILLE
- Giulia Gaglianone Lemos
- Marine Tetrapod Ecology and Conservation Laboratory, Marine Animal Stabilization Unit, São Francisco do Sul, University of the Joinville Region, UNIVILLE
- André Silva Barreto
- LIBGeo, UNIVALI
- Jeferson Luis Dick
- Marine Animal Stabilization Unit, University of Vale do Itajaí - Univali BR
- Karina Rejane Groch
- Australis Institute
- Pedro Volkmer de Castilho
- Zoology Laboratory, Stabilization Unit, Laguna, UDESC
- Deyvid Amgarten
- Albert Einstein Israelite Hospital
- Fernanda Malta
- Albert Einstein Israelite Hospital
- Michael Miller
- BSL-3 Laboratory of Virology and Applied Biotechnology, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas – UNICAMP
- Erick G. Dorlass
- Albert Einstein Israelite Hospital
- Soledad Palameta
- BSL-3 Laboratory of Virology and Applied Biotechnology, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas – UNICAMP
- Sun-Hak Lee
- Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University
- Clarice Weis Arns
- BSL-3 Laboratory of Virology and Applied Biotechnology, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas – UNICAMP
- Edison L. Durigon
- BSL3+ Laboratory of Clinical and Molecular Virology, Institute of Biomedical Sciences, University of São Paulo
- João Renato R. Pinho
- Albert Einstein Israelite Hospital
- Dong-Hun Lee
- Wildlife Health Laboratory, College of Veterinary Medicine, Konkuk University
- Helena Lage Ferreira
- Department of Veterinary Medicine, FZEA- USP, University of São Paulo
- DOI
- https://doi.org/10.1186/s12917-024-04137-1
- Journal volume & issue
-
Vol. 20,
no. 1
pp. 1 – 8
Abstract
Abstract Clade 2.3.4.4b highly pathogenic avian influenza (HPAI) H5N1 virus was detected in the South American sea lions found dead in Santa Catarina, Brazil, in October 2023. Whole genome sequencing and comparative phylogenetic analysis were conducted to investigate the origin, genetic diversity, and zoonotic potentials of the H5N1 viruses. The H5N1 viruses belonged to the genotype B3.2 of clade 2.3.4.4b H5N1 virus, which was identified in North America and disseminated to South America. They have acquired new amino acid substitutions related to mammalian host affinity. Our study provides insights into the genetic landscape of HPAI H5N1 viruses in Brazil, highlighting the continuous evolutionary processes contributing to their possible adaptation to mammalian hosts.
Keywords
- High pathogenicity avian influenza
- Whole genome sequencing
- Marine mammal
- South America
- Virus
- Public health
