Highly efficient RNA-guided base editing in rabbit

Zhiquan Liu; Mao Chen; Siyu Chen; Jichao Deng; Yuning Song; Liangxue Lai; Zhanjun Li

doi:10.1038/s41467-018-05232-2

Nature Communications (Jul 2018)

Highly efficient RNA-guided base editing in rabbit

Zhiquan Liu,
Mao Chen,
Siyu Chen,
Jichao Deng,
Yuning Song,
Liangxue Lai,
Zhanjun Li

Affiliations

Zhiquan Liu: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Mao Chen: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Siyu Chen: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Jichao Deng: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Yuning Song: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Liangxue Lai: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University
Zhanjun Li: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, Jilin University

DOI: https://doi.org/10.1038/s41467-018-05232-2
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

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Base editors can make targeted changes without inducing a double-stranded break. Here, the authors apply the BE3 and ABE7.10 systems to rabbit to create highly efficient targeted base substitutions and various mutation types, and show reduced frequency of undesired by-products with the updated BE4-Gam system.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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