Bioactive Materials (Jun 2024)
Self-adaptive pyroptosis-responsive nanoliposomes block pyroptosis in autoimmune inflammatory diseases
- Kaiwang Xu,
- Huang Yang,
- Jinghua Fang,
- Kaijie Qiu,
- Haotian Shen,
- Guanrui Huang,
- Qiangqiang Zheng,
- Canlong Wang,
- Tengjing Xu,
- Xinning Yu,
- Jiajie Wang,
- Yunting Lin,
- Jiacheng Dai,
- Yuting Zhong,
- Hongyun Song,
- Sunan Zhu,
- Siheng Wang,
- Zhuxing Zhou,
- Guang Yang,
- Zhengwei Mao,
- Zongyou Pan,
- Xuesong Dai
Affiliations
- Kaiwang Xu
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Huang Yang
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China
- Jinghua Fang
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Kaijie Qiu
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Haotian Shen
- Zhejiang University, Hangzhou, 310058, China
- Guanrui Huang
- Zhejiang University, Hangzhou, 310058, China
- Qiangqiang Zheng
- Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, 310000, China
- Canlong Wang
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Tengjing Xu
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Xinning Yu
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Jiajie Wang
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Yunting Lin
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Jiacheng Dai
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Yuting Zhong
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Hongyun Song
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Sunan Zhu
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Siheng Wang
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Zhuxing Zhou
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Guang Yang
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China
- Zhengwei Mao
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Department of Hepatobiliary and Pancreatic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Corresponding author. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
- Zongyou Pan
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China; Corresponding author. Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China.
- Xuesong Dai
- Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Orthopedics Research Institute, Zhejiang University, Hangzhou, 310009, China; Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, 310009, China; Clinical Research Center of Motor System Disease of Zhejiang Province, Hangzhou, 310009, China; Corresponding author. Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China.
- Journal volume & issue
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Vol. 36
pp. 272 – 286
Abstract
Nanoliposomes have a broad range of applications in the treatment of autoimmune inflammatory diseases because of their ability to considerably enhance drug transport. For their clinical application, nanoliposomes must be able to realize on-demand release of drugs at disease sites to maximize drug-delivery efficacy and minimize side effects. Therefore, responsive drug-release strategies for inflammation treatment have been explored; however, no specific design has been realized for a responsive drug-delivery system based on pyroptosis-related inflammation. Herein, we report a pioneering strategy for self-adaptive pyroptosis-responsive liposomes (R8-cardiolipin-containing nanoliposomes encapsulating dimethyl fumarate, RC-NL@DMF) that precisely release encapsulated anti-pyroptotic drugs into pyroptotic cells. The activated key pyroptotic protein, the N-terminal domain of gasdermin E, selectively integrates with the cardiolipin of liposomes, thus forming pores for controlled drug release, pyroptosis, and inflammation inhibition. Therefore, RC-NL@DMF exhibited effective therapeutic efficacies to alleviate autoimmune inflammatory damages in zymosan-induced arthritis mice and dextran sulfate sodium-induced inflammatory bowel disease mice. Our novel approach holds great promise for self-adaptive pyroptosis-responsive on-demand drug delivery, suppressing pyroptosis and treating autoimmune inflammatory diseases.
