Apolipoprotein A-I inhibits the increased activities of chitotriosidase and β-glucosaminidase in the liver of mice with BCG-induced tuberculosis inflammation

Abstract

The aim of the investigation was to study the activity of lysosomal chitinases (chitotriosidase and β-glucosaminidase) in the liver of mice using a model of BCG-induced tuberculous inflammation after intravenous administration of apolipoprotein A-I. Material and methods. The study was carried out on male CBA mice weighing 20–22 g. Disseminated tuberculous inflammation was modeled by a single intraperitoneal injection of 0.5 mg of BCG vaccine. The activity of chitinases was determined using fluorescent substrates 4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside and 4-methylumbelliferyl N-acetyl-β-D-glucosaminide. Results and discussion. BCG-infection of animals after 4 weeks caused a significant increase in the activity of endogenous chitinases in comparison with the control group: chitotriosidase – 3.05 times (p <0.001), β-glucosaminidase – 1.76 times (p <0.01). Intravenous administration of apolipoprotein A-I to animals against the background of BCG infection inhibited the increased enzyme activity, values did not significantly differ from the control values. Conclusions. The results of these studies indicate the ability of apolipoprotein A-I to reduce the increased activity of endogenous lysosomal chitinases in the liver of mice with BCGinduced tuberculous inflammation.

Keywords

• apolipoprotein a-i
• tuberculous inflammation
• bcg
• lysosomal chitinases