Selection of Explants and Optimization of Culture Conditions for Tissue Culture of Pholidota chinensis Lindl.

Abstract

【Objective】The study was carried out to establish a technical system suitable for tissue culture and rapid propagation of Pholidota chinensis Lindl.【Method】With the whole plant of P. chinensis Lindl as test material, the growth of explants was studied, the best explant and culture medium were selected, and the culture conditions such as seed germination and seedling proliferation were optimized.【Result】The best explant for tissue culture of P. chinensis Lindl. was the seed, and its roots, rhizomes and pseudobulbs did not germinate, but the seeds could germinate. The optimum culture formula of P. chinensis Lindl. was 1/2MS + 6-BA 1.5 mg/L + NAA 0.8 mg/L+6-KT 0.1 mg/L and the protocorm of P. chinensis Lindl. turned green under this condition. The organic matter selection of strong seedlings was conducted by adding banana puree (50 g/L), mashed potato (50 g/L) and coconut water (50 mL/L) to the basal medium, and it was found that the overall growth of P. chinensis Lindl. in the medium with banana puree was good and uniform with many pseudobulbs, and the plant height and leaf length were better than those of the P. chinensis Lindl. with mashed potato or coconut water added to the medium. Finally, 50 g/L banana puree was selected as the best organic matter added to the medium in the subsequent experiment. The best culture formula for the optimization of strong seedlings was 1/2MS + 50 g/L banana puree + 6-BA 0.5 mg/L + NAA 0.5 mg/L + 6-KT 0.3 mg/ L. Under this condition, the P. chinensis Lindl. grew well and the plant height was better. The best medium formula for rooting of strong seedlings was MS + 50 g/L banana puree + IBA 0.5 mg/L + NAA 0.5 mg/L. Under this condition, there were more pseudobulbs and more aerial roots on the seedlings.【Conclusion】To establish a technical system suitable for tissue culture and rapid propagation of P.chinensis Lindl. seeds, the best formula for the germination culture of P. chinensis Lindl. seeds is 1/2MS + 6-BA 1.5 mg/L + NAA 0.8 mg/L+6-KT 0.1 mg/L; the optimum culture formula for the optimization of strong seedlings is 1/2MS + 50 g/L banana puree + 6-BA 0.5 mg/L + NAA 0.5 mg/L + 6-KT 0.3 mg/L; the best medium formula for rooting is MS + 50 g/L banana puree + IBA 0.5 mg/L + NAA 0.5 mg/L. The study results lay a foundation for the resource conservation and utilization of P. chinensis Lindl. and its large-scale production.

Keywords

• pholidota chinensis lindl.
• tissue culture
• culture formula
• seed germination
• strong seedlings
• rooting