The Effects of Liraglutide on In Vitro Fertilization in Mice Following Experimental Diabetes

Abstract

Background and Objectives: Diabetes mellitus has adverse effects on reproductive system. Antidiabetic drugs are likely to develop impairment of fertility. In this regard, the present study was conducted to investigate the adverse effects of antidiabetic drug liraglutide on in vitro fertilization. Methods: A total of 48 adult female mice, were divided into 6 groups of 8 each, as follows: control group that did not receive the drug; non-diabetic group received liraglutide at a dose of 1.2 mg/kg BW; the non-diabetic group that was given liraglutide at a dose of 1.8 mg/kg BW; diabetic group that did not received liraglutide; diabetic group that received liraglutide at a dose of 1.2 mg/kg BW; and diabetic group that received that was given liraglutide at the dose of 1.8 mg/kg BW. Liraglutide was daily injected through subcutaneous route. After 35 days of treatment, the fertility potentials of the retrieved oocytes, was evaluated. Data were analyzed using one-way ANOVA and Tukey’s post hoc test. Results: In the diabetic group, a significant ­decrease (p < 0.05),­ were observed in the number of oocytes, percentages of fertilization, percentages of two-cell, four-cell, morula, blastocysts, and hatched embryos compared to the control group. In both diabetic groups receiving the drug, there was a decrease in the aforementioned parameters, but compared to the untreated diabetic group, there was an increase in these parameters. Conclusion: The results revealed that administration of liraglutide in diabetic mice improved fertility and increased the percentage of fertility and Embryos.

Keywords

• diabetes mellitus
• experimental
• fertilization in vitro
• liraglutide
• mice