Selection and valorization of arbuscular mycorrhizal fungi isolated from phosphate sludge basins in promoting of citrus and carob seedlings

Abstract

Accumulation of phosphate sludge (PS) generated from phosphate treatment process in the open air represent an environment risk and a problem in terms of storage capacities. The use of this by-products in agriculture, is an alternative recovery technique for the phosphate. Moreover, selection of symbionts and their inoculation into the soil whether in the nursery or at field were strongly encouraged. These inoculants were selected not only for their impact on the plant, but also for their ability to persist in the soil at the expense of the residual native microflora. This can be performed to the microorganisms which could occur in the solid sludges deriving the exploitation of phosphates in a pilot site of Khouribga area. In this context, the indigenous mycorrhizal resources have been exploited through better exploration of the local floristic diversity and then a series of mycorrhizal fungi selection and production tests have been made. The mycorrhizal complex was prepared and their effect was tested in Citrus and carob plants growth in the nursery. Two mixtures of PS were carried out: 10% (S2) and 40% (S5) for Citrus plants and 20% (S3) and 40% (S5) for carob plants. The sandy soil of Maamora forest was used as a control (S1). The results shown three dominant morphotypes were detected: Rhizophagus irregularis, Funnileformis constrictum and Scutellospora calospora. The effect of the PS with the AMF-based inoculum indicate that mycorrhizal inoculation of the substrates by a raw inoculum stimulates the growth of plants specially S2, S5 and S3 for Citrus volkameriana, Carrizo citrange and carob respectively. Based on these results, this combination between PS and mycorrhizal fungi had a great effect on Citrus and carob plant in the nursery.