Expression profile of lncRNA in miR-150 over-expressed cell line HK-2

Abstract

Objective To analyze differential expression of long non-coding RNA (lncRNA) after miR-150 over-expression by human renal tubular epithelial cell line HK-2 and explore the role of miR-150 in renal tubular injury through regulating the expression of lncRNA. Methods HK-2 cells with miR-150 mimic and scramble were sequenced by Illumina NovaSeq 6000 sequencer. The differentially expressed lncRNA expression profile, GO analysis and KEGG analysis were performed to verify miR-150-lncRNA-JAK2-STAT1/3-COL1 pathway. Results Compared with the scramble group, 362 differential expression lncRNAs were detected in miR-150 mimic group, of which, 252 lncRNAs were down-regulated and 110 lncRNAs were up-regulated both more than 1.5-fold. The results showed that the miR-150 regulated lncRNA and function in biological process, cell components and molecular mechanisms through enrichment analysis of KEGG pathway, miR-150-lncRNA-JAK2-STAT1/3-COL1 pathway was found and then verified by Western blot. Conclusions LncRNAs regulated by miR-150 may play some roles in pathogenesis and molecular mechanisms in human renal tubular injury.

Keywords

• renal tubular epithelial cell|mir-150|long noncoding rna|expression profile|hk-2