Frontiers in Cellular and Infection Microbiology (Dec 2020)

Human Short Peptidoglycan Recognition Protein PGLYRP1/Tag-7/PGRP-S Inhibits Listeria monocytogenes Intracellular Survival in Macrophages

  • Darya Slonova,
  • Darya Slonova,
  • Alexandra Posvyatenko,
  • Alexey Kibardin,
  • Elena Sysolyatina,
  • Elena Lyssuk,
  • Svetlana Ermolaeva,
  • Sergei Obydennyi,
  • Sergei Obydennyi,
  • Nikolay Gnuchev,
  • Georgii Georgiev,
  • Konstantin Severinov,
  • Sergey Larin

DOI
https://doi.org/10.3389/fcimb.2020.582803
Journal volume & issue
Vol. 10

Abstract

Read online

PGLYRP1/Tag-7/PGRP-S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag-7/PGRP-S potentiates the response of murine macrophage-like ANA-1 cells and human macrophages to facultative intracellular pathogen Listeria monocytogenes. PGLYRP1/Tag-7/PGRP-S binds to the surface of L. monocytogenes and other bacterial cells but has no effect on their growth in culture. While PGLYRP1/Tag-7/PGRP-S treatment modestly enhanced phagocytosis of bacteria by ANA-1 cells, the intracellular survival of PGLYRP1/Tag-7/PGRP-S treated L. monocytogenes was strongly inhibited 2 h after internalization. PGLYRP1/Tag-7/PGRP-S treatment of bacteria boosted oxidative burst induction and increased the level of proinflammatory cytokine IL-6 produced by ANA-1, however, these effects happened too late to be responsible for decreased intracellular survival of bacteria. Our results thus suggest that PGLYRP1/Tag-7/PGRP-S acts as a molecular sensor for detection of L. monocytogenes infection of mammalian cells that leads to increased killing through a mechanism(s) that remains to be defined.

Keywords